hplc

[amywy88]

Why is it good practice when setting up a HPLC experiment to record UV spectra not only of each analyte but also of the eluent? ( there are two parts to the answer, one to do with the molecules and solution and one to do with the instrument)

Really struggling with this question, any help would be greatly apprecited!!

[theprof]

Please don't take this the wrong way, but I think you need to go back to your notes on hplc or discuss this with a lecturer or hplc technician.

What you're asking here is pretty fundamental as far as hplc is concerned and you need to grasp these concepts before going any further.

I'm very sorry to be so unhelpful, but I can't give you a crash course on hplc on here and I feel you'd learn very little if I answered the question for you.