Firstly, if you are using timber t & g boards lay stretches of 5 boards and then cramp them together using floorboard cramps and then nailing them along the edge of each board with 3" losthead nails. Use a thick plank between the cramps and the boards to prevent damaging them.
Secondly, if you are using chipboard floor panels stagger the joints and screw them to the joists using 2" screws but I would not recommend gluing them to the joists or to each other because if plumbers or electricians need access at a later date you will regret it.