no any research you have saved to your tree will remain, you can still look at all the saved data and your tree in full. There are some indexed records that are free to look at (but not the primary source) and you won;t be able to contact other ancestry members without a subscription, but trust me, if you've done so well on that side you will want to do more, do what i do, I pay £10.95 a month for the monthly subscription, I use my debit card and it is quick and easy, you can also cancel a few days before the monthly reneqal if you don;t want to carry on.